GEN-AU

The Projects

Funded Projects


PORTRAITS OF SCIENTISTS

Microcosm for independent thinkers

The microbiologist Michael Wagner in portrait.

PORTRAITS OF SCIENTISTS

Rarely without communication problems

Political scientist Peter Biegelbauer portrayed by Sascha Karberg.
Managing Communicating

 
Austrian Network for Functional Mouse Genomics
This project is designed to establish a world-class technology platform for functional mouse genomics in Austria to rapidly translate genomic information into the context of a living organism and large scale validation of gene functions in normal physiology and disease in vivo.


The goal of the three year project is to establish an Austrian mouse genomics infrastructure. To achieve these aims, we have assembled a team of leading scientists and a network of principal Austrian institutes to provide and excel our infrastructure, to improve current technologies and to develop new technologies for large scale functional genome analysis. We will also combine and integrate our unique strength in novel RNAi technologies from other model systems (whole genome Drosophila RNAi library and large scale mammalian RNAi library) and basic RNAi biochemistry research with broad cutting edge mouse genetic technologies to bring Austria to the forefront of functional genomics research. Our approach to extend the latest knowledge of RNAi to a well characterized mammalian system should ultimately allow us to develop novel, large scale, and high-throughput methods in genetic engineering of mice. Thus, an RNAi platform with cross-references to other systems will be linked to genetically engineered mice to provide new and competitive tools for the genetic
engineering in a mammalian species. Path finding exemplary projects in defined diseases will act as the bench tests for our new approaches in analysis, management and experimental design for these novel technologies to generate systems for large scale analyses of gene function in a whole organism.


The multidisciplinary program has the following aims:


  • Development of state-of-the art animal facilities to strengthen and increase the efficacy and capacities of mouse genetic engineering technologies in Austria.

  • The establishment of a repository for genetically modified mice and genetic engineering services that will be made available to other Austrian researchers.

  • Development of imaging and telemetry technologies for in vivo mouse phenotyping.

  • Establishment of rapid and high through-put genetic engineering methods, in particular novel tetraploid ES cell and retroviral and targeted transgenesis.

  • Research in and development of novel RNAi systems for in vivo mouse use.

  • Development of unique genetic engineering techniques for genetic tissue-specific ON/OFF systems and site-specific integrations of target genes in the mouse genome.


I1 Depot für genetisch modifizierte Mäuse für Gewebsspezifisches Gentargeting: CRE Linien

Management:  Univ.-Prof. Dr. Josef Penninger

I2 Archivierung und Erhaltung von Mausmutanten

Management:  Univ. -Prof. Dr. Mathias Müller, Dr. Thomas Rülicke

I3 Phänotypisierung von Mausmutanten: MRI, Telemetrie, Kalorimetrie

Management:  Univ.-Prof. Dr. Josef Penninger, Erwin Wagner

T1 Entwicklung von RNA Interferenz (RNAi) Technologien in vivo

T1.1 Biochemical analysis of the RNAi pathway

Management:  Dr. Javier Martinez

T1.2 Genetische Analysen des RNAi Pfades

Management:  Dr. Barry Dickson

T1.3 RNAi in HeLa Zellen

Management:  Dr. Jan-Michael Peters

T2 Retrovirale und gezielte Transgene

T2.1 Generation von genetisch modifizierten Mäusen und Transgenes Service

Management:  Dr. Thomas Rülicke, Univ. -Prof. Dr. Mathias Müller

T2.2 Archivierung und Erhaltung von genetisch modifizierten Mäusen

Management:  Dr. Thomas Rülicke, Univ. -Prof. Dr. Mathias Müller

T2.3 Phänotypisierung von genetisch modifizierten Mäusen

Management:  Dr. Thomas Rülicke, Univ. -Prof. Dr. Mathias Müller

T3 Gentargeting Systeme

T3.1 Tetraploide Maustechnologie

Management:  Erwin Wagner, Univ.-Prof. Dr. Josef Penninger

T3.2 Etablierung einer schnellen und effizienten Transgenen Technologie

Management:  Maria Sibilia, Erwin Wagner

T3.3 Tet ON/OFF induzierbare Genexpression und homologe Chromsomen Inaktivierung in ES Zellen und Tetraploiden Mäusen in vivo.

Management:  Dr. Anton Wutz, Erwin Wagner


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