The goal of the three year project is to establish an Austrian mouse genomics infrastructure. To achieve these aims, we have assembled a team of leading scientists and a network of principal Austrian institutes to provide and excel our infrastructure, to improve current technologies and to develop new technologies for large scale functional genome analysis. We will also combine and integrate our unique strength in novel RNAi technologies from other model systems (whole genome Drosophila RNAi library and large scale mammalian RNAi library) and basic RNAi biochemistry research with broad cutting edge mouse genetic technologies to bring Austria to the forefront of functional genomics research. Our approach to extend the latest knowledge of RNAi to a well characterized mammalian system should ultimately allow us to develop novel, large scale, and high-throughput methods in genetic engineering of mice. Thus, an RNAi platform with cross-references to other systems will be linked to genetically engineered mice to provide new and competitive tools for the genetic
engineering in a mammalian species. Path finding exemplary projects in defined diseases will act as the bench tests for our new approaches in analysis, management and experimental design for these novel technologies to generate systems for large scale analyses of gene function in a whole organism.
The multidisciplinary program has the following aims:
- Development of state-of-the art animal facilities to strengthen and increase the efficacy and capacities of mouse genetic engineering technologies in Austria.
- The establishment of a repository for genetically modified mice and genetic engineering services that will be made available to other Austrian researchers.
- Development of imaging and telemetry technologies for in vivo mouse phenotyping.
- Establishment of rapid and high through-put genetic engineering methods, in particular novel tetraploid ES cell and retroviral and targeted transgenesis.
- Research in and development of novel RNAi systems for in vivo mouse use.
- Development of unique genetic engineering techniques for genetic tissue-specific ON/OFF systems and site-specific integrations of target genes in the mouse genome.